The attachment of the male-specific bacteriophage F1 to sensitive strains of Escherichia coli.
- 1 July 1966
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 56 (1), 126-132
- https://doi.org/10.1073/pnas.56.1.126
Abstract
In electron micrographs, fl was seen to attach by one end to the tip of F pili (filamentous appendages of male strains of E. coli with a diameter similar to that of the phage). The F pili were identified by their specific attachment of the spherical RNA phage MS-2 which adsorbs laterally along the entire length of the pilus. Phage fl can also attach to isolated F pili, but only at one of the two free ends of an F pilus fragment. That one end may contain several attachment ites, as shown by the attachment of two or even three fl bacteriophages. The DNA and RNA male-specific phages have completely distinct attachment sites. Attachment sites for fl are present along the entire length of an F pilus, but are only made available when the pilus is broken at any one point. Dimers and higher polymers of the phage attach in the same fashion.Keywords
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