Epidemiology and Control of Strawberry Bacterial Angular Leaf Spot Disease Caused by Xanthomonas fragariae
Open Access
- 1 August 2016
- journal article
- Published by Korean Society of Plant Pathology in The Plant Pathology Journal
- Vol. 32 (4), 290-299
- https://doi.org/10.5423/ppj.oa.01.2016.0007
Abstract
Strawberry bacterial angular leaf spot (ALS) disease, caused by Xanthomonas fragariae has become increasingly problematic in the strawberry agro-industry. ALS causes small angular water-soaked lesions to develop on the abaxial leaf surface. Studies reported optimum temperature conditions for X. fragariae are 20°C and the pathogen suffers mortality above 32°C. However, at the nursery stage, disease symptoms have been observed under high temperature conditions. In the present study, results showed X. fragariae transmission was via infected maternal plants, precipitation, and sprinkler irrigation systems. Systemic infections were detected using X. fragariae specific primers 245A/B and 295A/B, where 300-bp and 615-bp were respectively amplified. During the nursery stage (from May to August), the pathogen was PCR detected only in maternal plants, but not in soil or irrigation water through the nursery stage. During the cultivation period, from September to March, the pathogen was detected in maternal plants, progeny, and soil, but not in water. Additionally, un-infected plants, when planted with infected plants were positive for X. fragariae via PCR at the late cultivation stage. Chemical control for X. fragariae with oxolinic acid showed 87% control effects against the disease during the nursery period, in contrast to validamycin-A, which exhibited increased efficacy against the disease during the cultivation stage (control effect 95%). To our knowledge, this is the first epidemiological study of X. fragariae in Korean strawberry fields.Keywords
This publication has 18 references indexed in Scilit:
- Draft genome sequence of Xanthomonas fragariaereveals reductive evolution and distinct virulence-related gene contentBMC Genomics, 2013
- The Strawberry Plant Defense Mechanism: A Molecular ReviewPlant and Cell Physiology, 2011
- Low temperature and anhydrous electron microscopy techniques to observe the infection process of the bacterial pathogen Xanthomonas fragariae on strawberry leavesJournal of Microscopy, 2010
- Heat Treatment Effects on Strawberry Plant Survival and Angular Leaf Spot, Caused by Xanthomonas fragariae, in Nursery ProductionPlant Disease, 2009
- Development and Optimization of a Real-Time Detection Assay for Xanthomonas fragariae in Strawberry Crown Tissue with Receiver Operating Characteristic Curve AnalysisPhytopathology®, 2008
- Sensitive real‐time PCR detection of Xanthomonas fragariae in strawberry plantsPlant Pathology, 2008
- A rapid and sensitive method for the detection of Xanthomonas fragariae, causal agent of angular leafspot disease in strawberry plantsJournal of Microbiological Methods, 2004
- Bacterial leaf blight of strawberry (Fragaria (x) ananassa) caused by a pathovar of Xanthomonas arboricola, not similar to Xanthomonas fragariae Kennedy & King. Description of the causal organism as Xanthomonas arboricola pv. fragariae (pv. nov., comb. nov.)Plant Pathology, 2001
- Survival ofXanthomonas fragariaeon Strawberry in Summer Nurseries in Florida Detected by Specific Primers and Nested Polymerase Chain ReactionPlant Disease, 1996
- In vitro antibacterial activity of norfloxacin (MK-0366, AM-715) and other agents against gastrointestinal tract pathogensAntimicrobial Agents and Chemotherapy, 1983