Formation of Roseoflavin from 8-Amino- and 8-Methylamino-8-Demethyl-D-Riboflavin
- 1 March 1987
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 101 (3), 705-711
- https://doi.org/10.1093/jb/101.3.705
Abstract
A synthesis of roseoflavin (RoF) by Streptomyces davawensis from 8-amino- (AF) and 8-methylamino-8-demethyl-D-riboflavin (MAF) was demonstrated. The lines of evidence are: 1) the RoF formation was increased by addition of AF or MAF to the culture, 2) [2-14C]RoF was formed by addition of [2-14C]MAF or [2-14C]MAF to the culture, and the location of the 14C atom at the 2-position was demonstrated by identifying [14C]urea in the hydrolysate of the RoF, 3) [N-methyl-14C]RoF was formed by addition of [methyl-14C]methionine to the culture containing AF or MAF, and the location of the 14C atom was confirmed by the photochemical conversion of the RoF to MAF, the specific radioactivity of which was about half that of the original RoF, and by localization of the 14C atom in 1,2-dihydro-6-methyl-7-dimethylamino-2-keto-1-ribityl-3-quinoxalinecarboxylic acid (QC), which was formed from the RoF by hydrolysis.This publication has 3 references indexed in Scilit:
- Formation of Roseoflavin from Guanine through Riboflavin1The Journal of Biochemistry, 1979
- Anti-riboflavin activity of 8N-alkyl analogues of roseoflavin in some gram-positive bacteria.Journal of Nutritional Science and Vitaminology, 1978
- Mittheilungen aus dem organischen Laboratorium des Gewerbeinstitutes in Berlin: Untersuchungen über die HarnsäuregruppeEuropean Journal of Organic Chemistry, 1864