Structural determination and characterization of a 40 kDa protein isolated from rat 40 S ribosomal subunit
Open Access
- 28 February 1994
- journal article
- Published by Wiley in FEBS Letters
- Vol. 340 (1-2), 133-138
- https://doi.org/10.1016/0014-5793(94)80188-6
Abstract
We have purified a 40 kDa protein from the rat 40 S ribosomal subunit and determined its primary structure by amino acid and cDNA sequencing. The amino acid sequence of the 40 kDa protein shared 29–37% homology with prokaryotic ribosomal protein S2 of eubacteria and chloroplasts, indicating that the protein is a eukaryotic counterpart to prokaryotic S2. Moreover, the amino acid sequence shared 99% identity with those deduced from cDNAs for 68 kDa laminin binding proteins of human, murine and bovine origins. The cDNAs are capable of encoding polypeptides with predicted molecular mass of 33,000 which lacked typical signal sequences, N‐linked glycosylation sites and putative transmembrane domains. These results indicate that the eDNAs for 68 kDa laminin binding proteins actually code for the 40 kDa ribosomal protein.Keywords
This publication has 23 references indexed in Scilit:
- The primary structure of rat rig/ribosomal protein S15 geneBiochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1992
- Structural determination ofSaccharomyces cerevisiae riggene and identification of its product as ribosomal protein S21FEBS Letters, 1992
- rig encodes ribosomal protein S15 The primary structure of mammalian ribosomal protein S15FEBS Letters, 1991
- Characterization of a putative clone for the 67-kilodalton elastin/laminin receptor suggests that it encodes a cytoplasmic protein rather than a cell surface receptorBiochemistry, 1991
- Organization and nucleotide sequence of the genes for ribosomal protein S2 and elongation factor Ts inSpirulina PlatensisFEMS Microbiology Letters, 1990
- Evidence for a precursor of the high-affinity metastasis-associated murine laminin receptorBiochemistry, 1989
- Measurement of protein using bicinchoninic acidAnalytical Biochemistry, 1985
- Amino acid analysis by reverse-phase high-performance liquid chromatography: Precolumn derivatization with phenylisothiocyanateAnalytical Biochemistry, 1984
- [17] Isolation of microgram quantities of proteins from polyacrylamide gels for amino acid sequence analysisMethods in enzymology, 1983
- Primary structure of protein s2 from the Escherichia coli ribosomeFEBS Letters, 1981