Isolation of Trivittatus Virus from Larvae and Adults Reared from Field-Collected Larvae of Aedes Trivittatus (Diptera: Gulicidae)1

Abstract

Trivittatus virus (California group) was isolated from larvae and adult mosquitoes reared from field-collected larvae of Aedes trivittatus . Larvae collected in the spring were individuals that had overwintered in the egg stage. The isolation of trivittatus virus from 1st-brood larvae indicates that this virus overwinters in the eggs of Ae. trivittatus . The isolation of virus from 1st-brood adults demonstrates transstadial transmission as well. Trivittatus virus was isolated from 3 of 59 pools of larvae and from 4 of 36 pools of adult Ae. trivittatus reared from larvae. Two of 3 larval habitats examined contained larvae infected with this virus.