Three specimens of a new Pestalosphaeria species were obtained from a twig of Quercus myrsinaefolia from Kumamoto Pref., and from leaves of Rhododendron hybridum and Ricinus communis from Izu Peninsula, Shizuoka Pref. The collected materials were kept moist for 1 to 2 mo after collection. Monoascospore isolates from each specimen produced identical colonies with black slimy masses of conidia on them. Morphological characteristics of the conidia accorded well with those of Pestalotiopsis neglecta not only hitherto recorded but also formed on the same specimen. Hence, Pestalosphaeria gubae sp. nov. is proposed for the new species, as the teleomorph of Pestalotiopsis neglecta.