Plasma microRNAs serve as novel potential biomarkers for early detection of gastric cancer
- 10 January 2013
- journal article
- Published by Springer Science and Business Media LLC in Medical Oncology
- Vol. 30 (1), 1-7
- https://doi.org/10.1007/s12032-012-0452-0
Abstract
Recent studies have shown that microRNAs can be stably detected in human plasma and have the potential as non-invasive biomarkers for the diagnosis of cancers. This study evaluates the potential application of plasma microRNAs for the early detection of gastric cancer (GC). We first measured the plasma expression levels of 15 selected microRNAs (miR-1, -106a, -106b, -17-5p, -20a, -21, -221, -27a, -34, -376c, -378, -423-5p, -451, -486, -744) in 30 GC patients and 30 age- and gender-matched healthy controls and then validated those microRNAs that differentiating GC and controls in another 60 GC patients and 60 matched controls using quantitative reverse transcription–polymerase chain reaction. The areas under the receiver operating characteristic (ROC) curves were used to test the sensitivity and specificity of GC diagnosis using these identified plasma microRNAs. Three plasma microRNAs, miR-106b, miR-20a, and miR-221, were significantly elevated in GC patients than in healthy controls (P < 0.05). Furthermore, the areas under the ROC curves using miR-106b, miR-20a, and miR-221 for GC diagnosis were 0.7733 (95 % CI, 0.7758–0.8409), 0.8593 (95 % CI, 0.8046–0.9139), and 0.7960 (95 % CI, 0.7256–0.8664), respectively. Furthermore, these three microRNAs had a statistically significant elevation in GC patients compared with healthy controls at each of the four stages. However, there were no significant differences in the plasma levels of the three microRNAs among the four TNM stages (P > 0.05). Plasma miR-106b, miR-20a, and miR-221 have the potential as novel, non-invasive biomarkers for the early detection of GC.Keywords
This publication has 32 references indexed in Scilit:
- Prognostic value of a microRNA signature in nasopharyngeal carcinoma: a microRNA expression analysisThe Lancet Oncology, 2012
- Circulating MicroRNAs: a novel class of biomarkers to diagnose and monitor human cancersMedicinal Research Reviews, 2010
- Circulating microRNAs in plasma of patients with gastric cancersBritish Journal of Cancer, 2010
- Relation between microRNA expression and progression and prognosis of gastric cancer: a microRNA expression analysisThe Lancet Oncology, 2009
- Gastric cancerThe Lancet, 2009
- Characterization of microRNAs in serum: a novel class of biomarkers for diagnosis of cancer and other diseasesCell Research, 2008
- Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cellsNature, 2007
- Unique microRNA molecular profiles in lung cancer diagnosis and prognosisCancer Cell, 2006
- Molecular Classifiers for Gastric Cancer and Nonmalignant Diseases of the Gastric MucosaCancer Research, 2004
- Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT MethodMethods, 2001