Chemical and Functional Characterization of Italian Propolis Obtained by Different Harvesting Methods
- 12 March 2012
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of Agricultural and Food Chemistry
- Vol. 60 (11), 2852-2862
- https://doi.org/10.1021/jf205179d
Abstract
The composition and antioxidant activity of Italian poplar propolis obtained using three harvesting methods and extracted with different solvents were evaluated. Waxes, balsams, and resins contents were determined. Flavones and flavonols, flavanones and dihydroflavonols, and total phenolics were also analyzed. To characterize the phenolic composition, the presence of 15 compounds was verified through HPLC-MS/MS. The antioxidant activity was evaluated through 1,1-diphenyl-2-picrylhydrazyl radical and reducing power assays. The ability of propolis to inhibit lipid oxidation was monitored by analyzing hydroperoxide and TBARS formation in lipids incorporated into an oil-in-water (O/W) emulsion. Acetone shows the highest extraction capacity. Wedge propolis has the highest concentration of active phenolic compounds (TP = 359.1 ± 16.3 GAEs/g; TFF = 5.83 ± 0.42%; TFD = 7.34 ± 1.8%) and seems to be the most promising for obtaining high-value propolis more suitable to prepare high-quality dietary supplements (TBARS = 0.012 ± 0.009 mmol std/g; RP = 0.77 ± 0.07 TEs/g).Keywords
This publication has 34 references indexed in Scilit:
- 1H‐NMR Simultaneous Identification of Health‐Relevant Compounds in Propolis ExtractsPhytochemical Analysis, 2011
- Antioxidant Activity of Uruguayan Propolis. In Vitro and Cellular AssaysJournal of Agricultural and Food Chemistry, 2011
- From functional food to medicinal product: Systematic approach in analysis of polyphenolics from propolis and wineNutrition Journal, 2009
- Analysis of the polyphenolic fraction of propolis from different sources by liquid chromatography–tandem mass spectrometryJournal of Pharmaceutical and Biomedical Analysis, 2007
- Optimisation of an extraction procedure and chemical characterisation of Croatian propolis tincturesPhytochemical Analysis, 2007
- A Comparative Study on Phenolic Profiles and Antioxidant Activities of Legumes as Affected by Extraction SolventsJournal of Food Science, 2007
- Suppressive Effects of Ethanolic Extracts from Propolis and Its Main Botanical Origin on Dioxin ToxicityJournal of Agricultural and Food Chemistry, 2005
- Some Chemical Composition and Biological Activity of Northern Argentine PropolisJournal of Agricultural and Food Chemistry, 2005
- Isolation and Identification of Antimicrobial Compounds in Brazilian PropolisBioscience, Biotechnology, and Biochemistry, 1994
- Some Characteristics of the Enzymic Lipid Peroxidation System in the Microsomal Fraction of Flounder Skeletal MuscleJournal of Food Science, 1987