Characterization of Isolated Rat‐Liver Cells Made Permeable with Filipin
- 1 October 1981
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 119 (2), 409-414
- https://doi.org/10.1111/j.1432-1033.1981.tb05623.x
Abstract
When isolated rat-liver cells were incubated for 1 min at 37.degree. C with filipin at a concentration of 50 .mu.M, the plasma membrane became permeable to sucrose, inulin, glycerol 3-phosphate and other low MW compounds. Upon removal of the filipin and subsequent incubation of the cells at 37.degree. C there was a gradual leakage of lactate dehydrogenase from the cells. The leakage of lactate dehydrogenase could be prevented for about 10 min by including glutathione and ATP in the incubation medium. The filipin-treated cells were able to metabolize phosphorylated sugars. The conversion of fructose 1,6-bisphosphate to fructose 6-phosphate, G6-P and glucose was inhibited by AMP but not by high concentrations of fructose 1,6-bisphosphate. Filipin-treated cells may be used to study the kinetic parameters of enzymes in their macromolecular environment in situ.This publication has 48 references indexed in Scilit:
- In vitro Formation of Glucose 6-Phosphate from 3-Phosphoglycerate by Rat Liver CytosolHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1981
- In vitro formation of glucose-6-phosphate from glyceraldehyde-3-phosphate by liver cytosol from fed and starved rats. Effect of divalent cations on the conversion rateBiochemical and Biophysical Research Communications, 1979
- A general method for permeabilizing monolayer and suspension cultured animal cellsExperimental Cell Research, 1979
- Hepatic mitochondrial and cytosolic glutathione content and the subcellular distribution of GSH‐S‐transferasesFEBS Letters, 1979
- Reversible interconversions of sterol-binding and sterol-nonbinding forms of filipin as determined by fluorimetric and light scattertin propertiesBiochemistry, 1973
- Studies on the biological properties of polyene antibiotics: Comparison of other polyenes with filipin in their ability to interact specifically with sterolBiochimica et Biophysica Acta (BBA) - Biomembranes, 1972
- Fluorometric investigations of the interaction of polyene antibiotics with sterolsBiochemistry, 1972
- The dependence of phosphofructokinase kinetics upon protein concentrationFEBS Letters, 1971
- Enzyme Concentrations in TissuesScience, 1967
- Reversible inactivation and inhibition of liver fructose-1, 6-diphosphatase by adenosine nucleotidesBiochemical and Biophysical Research Communications, 1963