Reverse Phase High Pressure Liquid Chromatographic Determination of Aflatoxins in Foods
- 1 November 1978
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of AOAC INTERNATIONAL
- Vol. 61 (6), 1347-1352
- https://doi.org/10.1093/jaoac/61.6.1347
Abstract
A method for determining aflatoxins by high pressure liquid chromatography (HPLC) with fluorescence detection after CB extraction and cleanup has been applied to various foods. Recoveries at 1—15 ppb levels from green coffee and peanut butter were 72-85 and 74—104%, respectively. Precision of the method has been tested for peanut butter. Other products to which the method has been successfully applied include tree nuts, seeds, grains, chocolate-covered peanut butter candy, and roasted, salted-in- shell peanuts. High levels of aflatoxins found in several samples of nuts by this method have been verified by the official thin layer chromatographic (TLC) method. The advantages of this HPLC method are speed, precision, sensitivity, selectivity, and immediate chemical confirmation of aflatoxins B1 and G1. None of the products analyzed required special cleanup procedures. Preparative-scale HPLC was used to isolate purified B1 for toxicity testing.This publication has 6 references indexed in Scilit:
- Reverse Phase Analytical and Preparative High Pressure Liquid Chromatography of AflatoxinsJournal of AOAC INTERNATIONAL, 1977
- High Pressure Liquid Chromatographic Determination of Aflatoxins in Wines and Other Liquid ProductsJournal of AOAC INTERNATIONAL, 1977
- High Performance Liquid Chromatography of Aflatoxins in Cottonseed ProductsJournal of AOAC INTERNATIONAL, 1977
- Aflatoxin: Comparison of Analyses of Corn by Various MethodsJournal of AOAC INTERNATIONAL, 1977
- Reversed-phase high-performance liquid chromatographic analytical system for aflatoxins in wines with fluorescence detectionJournal of Chromatography A, 1977
- A Review of Sampling Plans and Collaboratively Studied Methods of Analysis for AflatoxinsJournal of AOAC INTERNATIONAL, 1976