Mutually exclusive interactions of EHD1 with GS32 and Syndapin II
- 9 July 2004
- journal article
- research article
- Published by Informa UK Limited in Molecular Membrane Biology
- Vol. 21 (4), 269-277
- https://doi.org/10.1080/09687680410001716871
Abstract
GS32/SNAP-29 is a SNAP-25-like SNARE and has been shown to interact with syntaxin 6. Using immobilized recombinant GS32, we have recovered EHD1 as a major GS32-interacting protein from total HeLa cell extracts. This interaction is mediated by the EH domain of EHD1 and the N-terminal NPF-containing 17-residue region of GS32. Co-immunoprecipitation suggests that GS32 could also interact with EHD1 in intact cells. When immobilized GST-EHD1 was used to fish out interacting proteins from total brain extracts, syndapin II was identified as a major interacting partner. Similar to the GS32-EHD1 interaction, syndapin II also interacts with the EH domain of EHD1 via its NPF repeat region. Interaction of endogenous EHD1 and syndapin II was also established by co-immunoprecipitation. Furthermore, interaction of GS32 and syndapin II with EHD1 was shown to be mutually exclusive, suggesting that EHD1 may regulate/participate in the functional pathways of both GS32 and syndapin II in a mutual exclusive manner. Opposing roles of GS32 and syndapin II in regulating the surface level of transferrin receptor (TfR) were observed.Keywords
This publication has 21 references indexed in Scilit:
- Syndapins integrate N-WASP in receptor-mediated endocytosisThe EMBO Journal, 2002
- The Eps15 homology (EH) domainFEBS Letters, 2001
- Rme-1 regulates the distribution and function of the endocytic recycling compartment in mammalian cellsNature, 2001
- Evidence that RME-1, a conserved C. elegans EH-domain protein, functions in endocytic recyclingNature, 2001
- Three ways to make a vesicleNature Reviews Molecular Cell Biology, 2000
- Accessory factors in clathrin-dependent synaptic vesicle endocytosisNature Reviews Neuroscience, 2000
- Syndapin Isoforms Participate in Receptor-Mediated Endocytosis and Actin OrganizationThe Journal of cell biology, 2000
- EHD2, EHD3, and EHD4 Encode Novel Members of a Highly Conserved Family of EH Domain-Containing ProteinsGenomics, 2000
- EHD1—An EH-Domain-Containing Protein with a Specific Expression PatternGenomics, 1999
- Syndapin I, a Synaptic Dynamin-binding Protein that Associates with the Neural Wiskott-Aldrich Syndrome ProteinMolecular Biology of the Cell, 1999