Bacterioplankton Community Shifts in an Arctic Lake Correlate with Seasonal Changes in Organic Matter Source
Open Access
- 1 April 2003
- journal article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 69 (4), 2253-2268
- https://doi.org/10.1128/aem.69.4.2253-2268.2003
Abstract
Seasonal shifts in bacterioplankton community composition in Toolik Lake, a tundra lake on the North Slope of Alaska, were related to shifts in the source (terrestrial versus phytoplankton) and lability of dissolved organic matter (DOM). A shift in community composition, measured by denaturing gradient gel electrophoresis (DGGE) of 16S rRNA genes, occurred at 4°C in near-surface waters beneath seasonal ice and snow cover in spring. This shift was associated with an annual peak in bacterial productivity ([ 14 C]leucine incorporation) driven by the large influx of labile terrestrial DOM associated with snow meltwater. A second shift occurred after the flux of terrestrial DOM had ended in early summer as ice left the lake and as the phytoplankton community developed. Bacterioplankton communities were composed of persistent populations present throughout the year and transient populations that appeared and disappeared. Most of the transient populations could be divided into those that were advected into the lake with terrestrial DOM in spring and those that grew up from low concentrations during the development of the phytoplankton community in early summer. Sequencing of DNA in DGGE bands demonstrated that most bands represented single ribotypes and that matching bands from different samples represented identical ribotypes. Bacteria were identified as members of globally distributed freshwater phylogenetic clusters within the α- and β- Proteobacteria , the Cytophaga-Flavobacteria-Bacteroides group, and the Actinobacteria .Keywords
This publication has 38 references indexed in Scilit:
- Dynamic bacterial and viral response to an algal bloom at subzero temperaturesLimnology and Oceanography, 2001
- Unusual bacterioplankton community structure in ultra‐oligotrophic Crater LakeLimnology and Oceanography, 2001
- Microbial population dynamics and diversity during a bloom of the marine coccolithophorid Emiliania huxleyi (Haptophyta)Marine Ecology Progress Series, 2001
- Microbial community dynamics in Mediterranean nutrient-enriched seawater mesocosms: changes in the genetic diversity of bacterial populationsFEMS Microbiology Ecology, 2001
- Integration of lakes and streams in a landscape perspective: the importance of material processing on spatial patterns and temporal coherenceFreshwater Biology, 2000
- The character and bioactivity of dissolved organic matter at thaw and in the spring runoff waters of the arctic tundra North Slope, AlaskaJournal of Geophysical Research: Solid Earth, 1998
- Bacterioplankton community structure: Protists control net production and the proportion of active bacteria in a coastal marine communityLimnology and Oceanography, 1996
- Effect of PCR conditions on the formation of heteroduplex and single-stranded DNA products in the amplification of bacterial ribosomal DNA spacer regions.Genome Research, 1993
- Nitrogen, Phosphorus, and Organic Carbon Cycling in an Arctic LakeCanadian Journal of Fisheries and Aquatic Sciences, 1985
- Seasonal Patterns of Bacterial Abundance in an Arctic LakeArctic and Alpine Research, 1983