Stability of biotransformation capacity in Digitalis lanata cell cultures after cryogenic storage
- 1 October 1983
- journal article
- Published by Springer Science and Business Media LLC in Plant Cell Reports
- Vol. 2 (5), 273-276
- https://doi.org/10.1007/bf00269159
Abstract
Suspension cultures of Digitalis lanata were stored at the temperature of liquid nitrogen. After thawing, viable cultures were recovered which showed a growth rate equal to that of untreated cells. The capacity of Digitalis cells to transform β-methyldigitoxin to β-methyldigoxin remained unchanged after cryostorage. This was shown in 300-ml shake cultures as well as in 20-1 bioreactors. From these results it is clear that cryostorage is a suitable method for preserving cell lines with specific capacities to produce compounds of medical and industrial importance.Keywords
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