Detection of S-phase Cell Cycle Progression using 5-ethynyl-2′-deoxyuridine Incorporation with Click Chemistry, an Alternative to using 5-bromo-2′-deoxyuridine Antibodies

Abstract
The 5-bromo-2′-deoxyuridine (BrdU) labeling of cells followed by antibody staining has been the standard method for direct measurement of cells in the S-phase. Described is an improved method for the detection of S-phase cell...