Nishihara Technique for the Solubilization of Collagen: Application to the Preparation of Soluble Collagens from Normal and Rheumatoid Connective Tissue
Open Access
- 1 July 1964
- journal article
- research article
- Published by BMJ in Annals Of The Rheumatic Diseases
- Vol. 23 (4), 300-301
- https://doi.org/10.1136/ard.23.4.300
Abstract
A method for the extraction of native soluble collagen from aged tendon was described. Pre-treatment of the tissue with [alpha]-amylase destroyed the covalent linkages which stabilized the connective tissue collagen, with the subsequent extraction of collagen in dilute acetic acid. This collagen is a highly polymerized form of tropocollagen with teleopeptides intact.Keywords
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