Isolation and Characterization of Two Flavonoids, Engeletin and Astilbin, from the Leaves of Engelhardia roxburghiana and Their Potential Anti-inflammatory Properties
- 19 April 2011
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of Agricultural and Food Chemistry
- Vol. 59 (9), 4562-4569
- https://doi.org/10.1021/jf2002969
Abstract
Engeletin, a flavonoid compound, was isolated from the leaves of Engelhardia roxburghiana for the first time, along with astilbin, another flavonoid. The chemical structures of engeletin and astilbin were confirmed by 1H and 13C nuclear magnetic resonance (NMR) and mass spectrometry (MS) spectra, and their anti-inflammatory activities were studied in lipopolysaccharide (LPS)-stimulated mouse J774A.1 macrophage cells. LPS induced the inflammatory state in macrophage cells and increased mRNA expressions of pro-inflammatory cytokines. Engeletin and astilbin exhibited remarkable inhibitory effects on interleukin (IL)-1β and IL-6 mRNA expression. Significant inhibition of LPS-mediated mRNA expressions were also seen in LPS binding toll-like receptor (TLR)-4, pro-inflammatory cytokine tumor necrosis factor (TNF)-α, IL-10, chemoattractant monocyte chemotactic protein (MCP)-1, and cyclooxygenase (COX)-2 genes. The reduced expression of these cytokines may alleviate immune response and reduce inflammatory activation, indicating that engeletin and astilbin may serve as potential anti-inflammatory agents.Keywords
This publication has 45 references indexed in Scilit:
- Oxidative stress, inflammation, and cancer: How are they linked?Free Radical Biology & Medicine, 2010
- Anti-Inflammatory and Antiproliferative Activities of Trifolirhizin, a Flavonoid from Sophora flavescens RootsJournal of Agricultural and Food Chemistry, 2009
- Interleukin-6 (IL-6) released by macrophages induces IL-6 secretion in the human colon cancer HT-29 cell lineHuman Immunology, 2009
- Boric acid inhibits LPS-induced TNF-α formation through a thiol-dependent mechanism in THP-1 cellsJournal of Trace Elements in Medicine and Biology, 2008
- Systematic review of the association between circulating interleukin-6 (IL-6) and cancerEuropean Journal Of Cancer, 2008
- Differential effects of resveratrol on androgen-responsive LNCaP human prostate cancer cells in vitro and in vivoCarcinogenesis: Integrative Cancer Research, 2008
- Expression of COX-1 and COX-2 in a Clinical Model of Acute InflammationThe Journal of Pain, 2007
- Pathogen Recognition and Innate ImmunityCell, 2006
- Inflammation and cancerNature, 2002
- Sweet dihydroflavonol rhamnoside from leaves of Engelhardtia chrysolepis, a chinese folk medicine, hung-qi.CHEMICAL & PHARMACEUTICAL BULLETIN, 1988