In‐cell protein NMR and protein leakage
- 11 October 2010
- journal article
- research article
- Published by Wiley in Proteins-Structure Function and Bioinformatics
- Vol. 79 (2), 347-351
- https://doi.org/10.1002/prot.22906
Abstract
In‐cell nuclear magnetic resonance spectroscopy is a tool for studying proteins under physiologically relevant conditions. In some instances, however, protein signals from leaked protein are observed in the liquid surrounding the cells. Here, we examine the expression of four proteins in Escherichia coli. We describe the controls that should be used for in‐cell NMR experiments and show that leakage is likely when the protein being studied exceeds ∼20% of the total cellular protein. Proteins 2011.Keywords
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