Blood CD34−c-Kit+ cell rate correlates with aggressive forms of systemic mastocytosis and behaves like a mast cell precursor
- 10 November 2011
- journal article
- research article
- Published by American Society of Hematology in Blood
- Vol. 118 (19), 5246-5249
- https://doi.org/10.1182/blood-2011-02-335950
Abstract
Mastocytosis is a heterogeneous disease characterized by the accumulation of mast cells in one or more organs. Our objective was to identify a peripheral mast cell precursor and assess its variation rate in mastocytosis. A peripheral blood phenotypic analysis was performed among 50 patients with mastocytosis who were enrolled in a prospective multicentric French study, and the phenotypic analysis results of the patients were compared with those of healthy donors. The rate of peripheral blood CD34−c-Kit+ cells correlated with the severity of mastocytosis. This cellular population was isolated from healthy donors as well as from patients with systemic mastocytosis. After 30 days of culture, the CD34−c-Kit+ cells gave birth to mature mast cells, indicating that this cellular population constitutes a mast cell circulating precursor. Monitoring peripheral CD34−c-Kit+ cells by flow cytometry could be a useful and low-invasive tool to determine the disease severity and the relapses and to assess treatment efficiency.Keywords
This publication has 21 references indexed in Scilit:
- Mast cells from different molecular and prognostic subtypes of systemic mastocytosis display distinct immunophenotypesJournal of Allergy and Clinical Immunology, 2010
- Pediatric Mastocytosis Is a Clonal Disease Associated with D816V and Other Activating c-KIT MutationsJournal of Investigative Dermatology, 2010
- Prognostically relevant breakdown of 123 patients with systemic mastocytosis associated with other myeloid malignanciesBlood, 2009
- Systemic mastocytosis in 342 consecutive adults: survival studies and prognostic factorsBlood, 2009
- Masitinib is Safe and Effective for the Treatment of Canine Mast Cell TumorsJournal of Veterinary Internal Medicine, 2008
- Immunophenotypic analysis of mast cells in mastocytosis: When and how to do it. Proposals of the Spanish Network on Mastocytosis (REMA)Cytometry Part B: Clinical Cytometry, 2004
- Utility of flow cytometric analysis of mast cells in the diagnosis and classification of adult mastocytosisLeukemia Research, 2001
- Classification and Diagnosis of Mastocytosis: Current StatusJournal of Investigative Dermatology, 1991
- A high-sampling-rate automated continuous-flow fluorometric technique for the analysis of nanogram levels of histamine in biological samplesAnalytical Biochemistry, 1983
- Mast cells and mast cell neoplasia: a review*Histopathology, 1979