Sulfated N‐linked carbohydrate chains in porcine thyroglobulin
- 5 December 1988
- journal article
- Published by Wiley in FEBS Letters
- Vol. 241 (1-2), 246-250
- https://doi.org/10.1016/0014-5793(88)81070-6
Abstract
N-linked carbohydrate chains of porcine thyroglobulin were released by the hydrazinolysis procedure. The resulting mixture of oligosaccharide-alditols was fractionated by high-voltage paper electrophoresis, the acidic fractions were further separated by high-performance liquid chromatography on Lichrosorb-NH2, and analyzed by 500-MHz 1H-NMR spectroscopy and, partially, by permethylation analysis. Of the acidic oligosaccharide-alditols, the following sulfated carbohydrate chains could be identified:Keywords
This publication has 33 references indexed in Scilit:
- 1H- and 13C-n.m.r. spectroscopy of synthetic monosulphated methyl α-d-galactopyranosidesCarbohydrate Research, 1988
- Primary structure of the acidic carbohydrate chain of hemocyanin from Panulirus interruptusFEBS Letters, 1987
- Determination of the structure of sulfated tetra- and pentasaccharides obtained by alkaline borohydride degradation of hen ovomucin. A fast atom bombardment-mass spectrometric and1H-NMR spectroscopic studyGlycoconjugate Journal, 1987
- 1H-NMR studies at 500 MHz of a neutral disaccharide and sulphated di-, tetra-, hexa- and larger oligosaccharides obtained by endo-beta-galactosidase treatment of keratan sulphateEuropean Journal of Biochemistry, 1986
- Phosphorylated high mannose-type and hybrid-type oligosaccharide chains of human thyroglobulin isolated from malignant thyroid tissueBiochimica et Biophysica Acta (BBA) - General Subjects, 1984
- Structural changes of carbohydrate chains of human thyroglobulin accompanying malignant transformations of thyroid glandsEuropean Journal of Biochemistry, 1984
- The identification of terminal α(1→3)-linked galactose in N-acetyllactosamine type of glycopeptides by means of 500-MHz 1H-NMR spectroscopyBiochemical and Biophysical Research Communications, 1984
- Primary structure of the major glycans of the N‐acetyllactosamine type derived from the human immunoglobulins M from two patients with Waldenström's macroglobulinemiaFEBS Letters, 1984
- Determination, by methylation analysis, of the glycosyl-linkage compositions of microgram quantities of complex carbohydratesCarbohydrate Research, 1983
- A high-pressure liquid chromatography method for the detection of O-β-d-galactosyl-(1→4)-2-acetamido-2-deoxy-d-glucose α-(2→3)- and α-(2→6)-sialyltransferasesCarbohydrate Research, 1983