Interaction of oral bacteria with gingival epithelial cell multilayers
- 28 March 2011
- journal article
- Published by Wiley in Molecular Oral Microbiology
- Vol. 26 (3), 210-220
- https://doi.org/10.1111/j.2041-1014.2011.00609.x
Abstract
Primary gingival epithelial cells were cultured in multilayers as a model for the study of interactions with oral bacteria associated with health and periodontal disease. Multilayers maintained at an air–liquid interface in low‐calcium medium displayed differentiation and cytokeratin properties characteristic of junctional epithelium. Multilayers were infected with fluorescently labeled Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum or Streptococcus gordonii, and bacterial association was determined by confocal microscopy and quantitative image analysis. Porphyromonas gingivalis invaded intracellularly and spread from cell to cell; A. actinomycetemcomitans and F. nucleatum remained extracellular and showed intercellular movement through the multilayer; whereas S. gordonii remained extracellular and predominantly associated with the superficial cell layer. None of the bacterial species disrupted barrier function as measured by transepithelial electrical resistance. P. gingivalis did not elicit secretion of proinflammatory cytokines. However, A. actinomycetemcomitans and S. gordonii induced interleukin‐1β (IL‐1β), tumor necrosis factor‐α (TNF‐α), IL‐6 and IL‐8 secretion; and F. nucleatum stimulated production of IL‐1β and TNF‐α. Aggregatibacter actinomycetemcomitans, F. nucleatum and S. gordonii, but not P. gingivalis, increased levels of apoptosis after 24 h infection. The results indicate that the organisms with pathogenic potential were able to traverse the epithelium, whereas the commensal bacteria did not. In addition, distinct host responses characterized the interaction between the junctional epithelium and oral bacteria.Keywords
This publication has 50 references indexed in Scilit:
- Anti- Porphyromonas gingivalis and Anti-Inflammatory Activities of A-Type Cranberry ProanthocyanidinsAntimicrobial Agents and Chemotherapy, 2010
- Aggregatibacter actinomycetemcomitansLeukotoxinJournal of Dental Research, 2010
- Epithelial cell pro‐inflammatory cytokine response differs across dental plaque bacterial speciesJournal of Clinical Periodontology, 2009
- Bacterial invasion of epithelial cells and spreading in periodontal tissuePeriodontology 2000, 2009
- Subgingival biofilm formationPeriodontology 2000, 2009
- StreptococcusAdherence and ColonizationMicrobiology and Molecular Biology Reviews, 2009
- Porphyromonas gingivalis–host interactions: open war or intelligent guerilla tactics?Microbes and Infection, 2009
- Quantitative proteomics of intracellular Porphyromonas gingivalisProteomics, 2007
- Gingival Epithelial Cell Transcriptional Responses to Commensal and Opportunistic Oral Microbial SpeciesInfection and Immunity, 2007
- Intrinsic apoptotic pathways of gingival epithelial cells modulated by Porphyromonas gingivalisCellular Microbiology, 2007