Gene Expression of Antioxidative Enzymes in the Human Heart
- 4 January 2000
- journal article
- other
- Published by Wolters Kluwer Health in Cell Metabolism
- Vol. 101 (1), 33-39
- https://doi.org/10.1161/01.cir.101.1.33
Abstract
Background—An increase in oxidative stress is suggested to be intimately involved in the pathogenesis of heart failure. However, gene expression of enzymes that metabolize reactive oxygen metabolites has not been investigated in the human heart. Methods and Results—Myocardial tissue homogenates of the left ventricular wall from hearts in end-stage failure due to dilated (DCM) or ischemic (ICM) cardiomyopathy (n=12 each), as well as from nonfailing donor hearts (n=12), were analyzed for mRNA levels of manganese superoxide dismutase (MnSOD), copper-zinc superoxide dismutase (CuZnSOD), glutathione peroxidase (GPX), and catalase by Northern blot analyses. Protein levels of MnSOD, CuZnSOD, and catalase were determined by Western blot or ELISA. MnSOD, CuZnSOD, and GPX mRNA levels were similar in all 3 groups. In contrast, catalase mRNA levels were found to be increased by 123±23% in DCM hearts and by 93±10% in ICM hearts (PPPConclusions—Increased oxidative stress in human end-stage heart failure may result in a specific upregulation of catalase gene expression as a compensatory mechanism, whereas SOD and GPX gene expression remain unaffected.Keywords
This publication has 34 references indexed in Scilit:
- In VitroAntiarrhythmic Effect of Prior Whole Body Hyperthermia: Implication of CatalaseJournal of Molecular and Cellular Cardiology, 1997
- Tissue differences in antioxidant enzyme gene expression in response to endotoxinFree Radical Biology & Medicine, 1996
- Oxy free radical system in heart failure and therapeutic role of oral vitamin EInternational Journal of Cardiology, 1996
- Chronic Exposure of Neonatal Cardiac Myocytes to Hydrogen Peroxide Enhances the Expression of CatalaseJournal of Molecular and Cellular Cardiology, 1996
- Oxidative stress and heart failureMolecular and Cellular Biochemistry, 1995
- The potential for endogenous myocardial antioxidants to protect the mycocardium against ischaemia-reperfusion injury: Refreshing the parts exogenous antioxidants cannot reach?Journal of Molecular and Cellular Cardiology, 1995
- Purification and characterization of glutathione peroxidase from human blood platelets. Age-related changes in the enzymeBiochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, 1994
- The free radical system in ischemic heart diseaseInternational Journal of Cardiology, 1994
- Elevated breath pentane in heart failure reduced by free radical scavengerFree Radical Biology & Medicine, 1993
- Isolation and characterization of complementary DNAs encoding human manganese‐containing superoxide dismutaseFEBS Letters, 1988