Cloning and Nucleotide Sequences of the Topoisomerase IV parC and parE Genes of Mycoplasma hominis
- 1 August 1998
- journal article
- research article
- Published by American Society for Microbiology in Antimicrobial Agents and Chemotherapy
- Vol. 42 (8), 2024-2031
- https://doi.org/10.1128/aac.42.8.2024
Abstract
The topoisomerase IV parC and parE genes from the wall-less organism Mycoplasma hominis PG21 were cloned and sequenced. The coupled genes are located far from the DNA gyrase genes gyrA and gyrB . They encode proteins of 639 and 866 amino acids, respectively. As expected, the encoded ParE and ParC proteins exhibit higher homologies with the topoisomerase IV subunits of the gram-positive bacteria Staphylococcus aureus and Streptococcus pneumoniae than with their Escherichia coli counterparts. The conserved regions include the Tyr residue of the active site and the region involved in quinolone resistance (quinolone resistance-determining region [QRDR]) in ParC and the ATP-binding site and the QRDR in ParE.Keywords
This publication has 48 references indexed in Scilit:
- AnnouncementFEMS Microbiology Reviews, 1996
- New genes in the 170 region of the Bacillus subtilis genome encode DNA gyrase subunits, a thioredoxin, a xylanase and an amino acid transporterMicrobiology, 1996
- The unique DNA topology and DNA topoisomerases of hyperthermophilic archaeaFEMS Microbiology Reviews, 1996
- Neisseria gonorrhoeae acquires mutations in analogous regions of gyrA and parC in fluoroquinolone‐resistant isolatesMolecular Microbiology, 1994
- The role of topoisomerase IV in partitioning bacterial replicons and the structure of catenated intermediates in DNA replicationCell, 1992
- Basic Local Alignment Search ToolJournal of Molecular Biology, 1990
- Basic local alignment search toolJournal of Molecular Biology, 1990
- Mycoplasma pneumoniae DNA gyrase genesMolecular Microbiology, 1990
- Cloning and sequencing of the Escherichia coli gyrA gene coding for the a subunit of DNA gyraseJournal of Molecular Biology, 1987
- Studies on transformation of Escherichia coli with plasmidsJournal of Molecular Biology, 1983