Micropatterned Aptasensors for Continuous Monitoring of Cytokine Release from Human Leukocytes

Abstract

We report the development of a microdevice for detecting local interferon gamma (IFN-γ) release from primary human leukocytes in real time. Our microdevice makes use of miniature aptamer-modified electrodes integrated with microfluidics to monitor cellular production of IFN-γ. The aptamer species consists of a DNA hairpin molecule with thiol groups on the 3′-end for self-assembly onto Au electrodes. A redox reporter is covalently attached at the 5′-end for electrochemical sensing. This aptasensor has excellent sensitivity for IFN-γ (–1 h^–1 upon activation. The work described here represents an important step toward development of aptasensors for immune cell analysis and blood-based diagnostics.