Detection of fetal RhD sequence from peripheral blood of sensitized RhD‐negative pregnant women
- 1 July 1994
- journal article
- Published by Wiley in British Journal of Haematology
- Vol. 87 (3), 658-660
- https://doi.org/10.1111/j.1365-2141.1994.tb08336.x
Abstract
A sensitive PCR-based assay was developed to amplify fetal-derived rhesus D (RhD) sequence from peripheral blood of RhD-negative pregnant women with circulating anti-D. RhD-PCR positivity was detected in 7/22 samples from women bearing RhD-positive fetuses, despite the presence of varying levels of anti-D. Evidence is presented which suggests that rising maternal anti-D levels might reduce circulating fetal cell numbers. Further development of this assay may have implications in the clinical management of RhD-sensitized pregnancies and aid the understanding of the physiology of feto-maternal cell trafficking.Keywords
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