Endothelial nitric oxide synthase in hypoxic newborn porcine pulmonary vessels
Open Access
- 1 July 1997
- journal article
- Published by BMJ in Archives of Disease in Childhood: Fetal & Neonatal
- Vol. 77 (1), F16-F22
- https://doi.org/10.1136/fn.77.1.f16
Abstract
AIMS To determine if the failure of neonatal pulmonary arteries to dilate is due to a lack of nitric oxide synthase (NOS). METHODS A monoclonal antibody to endothelial NOS was used to demonstrate the distribution and density of NOS in the developing porcine lung after a period in hypobaric hypoxia. Newborn piglets were made hypertensive by exposure to hypobaric hypoxia (50.8 kPa) from < 5 minutes of age to 2.5 days of age, 3–6 days of age or 14–17 days of age. A semiquantitative scoring system was used to assess the distribution of endothelial NOS by light microscopy. RESULTS NOS was present in the arteries in all hypoxic animals. However, hypoxia from birth caused a reduction in NOS compared with those lungs normal at birth and those normal at 3 days. Hypoxia from 3–6 days led to a high density of NOS compared with normal lungs at 6 days. Hypoxia from 14–17 days had little effect on the amount of NOS. On recovery in room air after exposure to hypoxia from birth there was a transient increase in endothelial NOS after three days of recovery, mirroring that seen at three days in normal animals. CONCLUSIONS Suppression of NOS production in the first few days of life may contribute to pulmonary hypertension in neonates.Keywords
This publication has 23 references indexed in Scilit:
- Inhaled nitric oxide in persistent pulmonary hypertension of the newbornThe Lancet, 1992
- Purification and characterization of particulate endothelium-derived relaxing factor synthase from cultured and native bovine aortic endothelial cells.Proceedings of the National Academy of Sciences, 1991
- Loss of endothelium-dependent relaxant activity in the pulmonary circulation of rats exposed to chronic hypoxia.JCI Insight, 1991
- Pulmonary vascular damage and the development of cor pulmonale following hyaline membrane diseasePediatric Pulmonology, 1990
- The glucose oxidase-DAB-nickel method in peroxidase histochemistry of the nervous systemNeuroscience Letters, 1988
- Impaired adaptation of pulmonary circulation to extrauterine life in newborn pigs exposed to hypoxia: An ultrastructural studyThe Journal of Pathology, 1986
- Normal adaptation of pulmonary arterial intima to extrauterine life in the pig: Ultrastructural studiesThe Journal of Pathology, 1986
- Effect of hypoxia on adaptation of the pulmonary circulation to extra-uterine life in the pigCardiovascular Research, 1982
- Small pulmonary arteries in some natives of La Paz, Bolivia.Thorax, 1981
- ENDOTHELIAL AND SUBINTIMAL CHANGES IN RAT HILAR PULMONARY-ARTERY DURING RECOVERY FROM HYPOXIA - A QUANTITATIVE ULTRASTRUCTURAL-STUDY1980