Transformation of human ciliary epithelial cells by simian virus 40: induction of cell proliferation and retention of beta 2-adrenergic receptors.
- 1 November 1986
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 83 (22), 8754-8758
- https://doi.org/10.1073/pnas.83.22.8754
Abstract
Ciliary epithelial cells derived from human eye were successfully propagated through many generations after transformation with simian virus 40. The cell clone 8-SVHCE was isolated and characterized by immunoprecipitation and pharmacological studies that demonstrated the presence of several functional properties observed in the parent cells of this tissue. Immunoprecipitation revealed the presence of large tumor (T) antigen, and Southern blot analysis showed the incorporation of viral DNA into high molecular weight ciliary epithelial cell DNA. The presence of .beta.-adrenergic receptors was demonstrated by direct binding of a radiolabeled antagonist, [125I]iodopindolol, to membrane preparations of 8-SVHCE cells (Kd = 41.8 pM and Bmax = 67.1 fmol/mg of protein). Competition experiments with [125I]iodopindolol and selective drugs suggested that the receptors are of the .beta.2-adrenergic subtype. Studies of catecholamine-stimulated cellular cAMP production and of isoproterenol-dependent protein phosphorylation of vimentin in 8-SVHCE indicated the functional conversation of .beta.-adrenergic receptor-mediated processes that are thought to be important in the regulation of aqueous humor production by the ciliary epithelium in vivo.This publication has 30 references indexed in Scilit:
- Beta-adrenergic receptors in ciliary processes of the rabbit.1980
- (-)-[I-125-LABELED IODOPINDOLOL, A NEW HIGHLY SELECTIVE RADIOIODINATED BETA-ADRENERGIC-RECEPTOR ANTAGONIST - MEASUREMENT OF BETA-RECEPTORS ON INTACT RAT ASTROCYTOMA-CELLS1980
- INVITRO DETERMINATION OF ABILITY OF DRUGS TO BIND TO ADRENERGIC-RECEPTORS1977
- Monomer molecular weight of T antigen from simian virus 40-infected and transformed cells.Proceedings of the National Academy of Sciences of the United States of America, 1976
- Detection of specific sequences among DNA fragments separated by gel electrophoresisJournal of Molecular Biology, 1975
- Relationship between the inhibition constant (KI) and the concentration of inhibitor which causes 50 per cent inhibition (I50) of an enzymatic reactionBiochemical Pharmacology, 1973
- Radioimmunoassay for cyclic nucleotides. I. Preparation of antibodies and iodinated cyclic nucleotides.1972
- Some characteristics of an adenyl cyclase preparation from rabbit ciliary process tissueExperimental Eye Research, 1971
- A comparative study of the autonomic innervation of the eye in monkey, cat, and rabbitThe Anatomical Record, 1966
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951