Immunoreactive peptide maps of SARS-CoV-2
Open Access
- 12 February 2021
- journal article
- research article
- Published by Springer Science and Business Media LLC in Communications Biology
- Vol. 4 (1), 1-7
- https://doi.org/10.1038/s42003-021-01743-9
Abstract
Serodiagnosis of SARS-CoV-2 infection is impeded by immunological cross-reactivity among the human coronaviruses (HCoVs): SARS-CoV-2, SARS-CoV-1, MERS-CoV, OC43, 229E, HKU1, and NL63. Here we report the identification of humoral immune responses to SARS-CoV-2 peptides that may enable discrimination between exposure to SARS-CoV-2 and other HCoVs. We used a high-density peptide microarray and plasma samples collected at two time points from 50 subjects with SARS-CoV-2 infection confirmed by qPCR, samples collected in 2004-2005 from 11 subjects with IgG antibodies to SARS-CoV-1, 11 subjects with IgG antibodies to other seasonal human coronaviruses (HCoV), and 10 healthy human subjects. Through statistical modeling with linear regression and multidimensional scaling we identified specific peptides that were reassembled to identify 29 linear SARS-CoV-2 epitopes that were immunoreactive with plasma from individuals who had asymptomatic, mild or severe SARS-CoV-2 infections. Larger studies will be required to determine whether these peptides may be useful in serodiagnostics. Mishra, Huang et al. identify 29 linear SARS-CoV-2 epitopes that are immunoreactive with the plasma from individuals who had asymptomatic, mild, or severe SARS-CoV-2 infections. This study suggests the possibility of using these peptides to discriminate the exposure to SARS-CoV-2 and other human coronaviruses.Keywords
This publication has 24 references indexed in Scilit:
- Peptide microarray-based characterization of antibody responses to host proteins after bacille Calmette–Guérin vaccinationInternational Journal of Infectious Diseases, 2017
- Robust hyperparameter estimation protects against hypervariable genes and improves power to detect differential expressionThe Annals of Applied Statistics, 2016
- Proteome-wide Epitope Mapping of Antibodies Using Ultra-dense Peptide ArraysMolecular & Cellular Proteomics, 2014
- High-resolution Mapping of Linear Antibody Epitopes Using Ultrahigh-density Peptide MicroarraysMolecular & Cellular Proteomics, 2012
- edgeR: a Bioconductor package for differential expression analysis of digital gene expression dataBioinformatics, 2009
- IntroductionPublished by Springer Nature ,2009
- ggplot2Published by Springer Science and Business Media LLC ,2009
- [Study on the dynamic prevalence of serum antibody against severe acute respiratory syndrome coronavirus in employees from wild animal market in Guangzhou].2006
- Prevalence of IgG antibody to SARS-associated coronavirus in animal traders--Guangdong Province, China, 2003.2003
- A comparison of normalization methods for high density oligonucleotide array data based on variance and biasBioinformatics, 2003