Serological analysis of the subgroup protein of rotavirus, using monoclonal antibodies

Abstract

Mouse monoclones directed to the 42,000-dalton inner sturctural protein of rotavirus were analyzed. Eight monoclones reacted broadly with antigenic domains common to virtually all mammalian rotaviruses. Two monoclones had specificities similar or identical to previously characterized subgroup specificities. These subgroup monoclones were more efficient in detecting subgroup antigen than either hyperimmune or postinfection antisera. Using the subgroup monoclones, some animal and human rotavirus strains were found to carry subgroup 2 specificity and epizootic diarrhea of infant mice virus and turkey rotavirus were shown to be antigenically distinct from other mammalian rotavirus strains.