The Pattern of Protein Synthesis Induced by Heat Shock of HeLa Cells

Abstract

While incubation of HeLa cells for 5-10 min at 45.degree. C does not affect subsequent cellular growth and DNA synthesis at 37.degree. C, there is an increased synthesis of specific proteins in the MW region of 100,000, 72,000-74,000 and 37,000. The synthesis of these proteins reaches a maximum 2 h after the heat shock. The proteins themselves are stable, but their synthesis declines to normal levels 4 h afterwards. The increased synthesis is blocked by actinomycin D but the addition of cycloheximide or NaF during the induction period is without effect.