Monitoring of gene knockouts: genome-wide profiling of conditionally essential genes
Open Access
- 1 January 2007
- journal article
- Published by Springer Science and Business Media LLC in Genome Biology
- Vol. 8 (5), R87
- https://doi.org/10.1186/gb-2007-8-5-r87
Abstract
We have developed a new microarray-based genetic technique, named MGK (Monitoring of Gene Knockouts), for genome-wide identification of conditionally essential genes. MGK identified bacterial genes that are critical for fitness in the absence of aromatic amino acids, and was further applied to identify genes whose inactivation causes bacterial cell death upon exposure to the bacteriostatic antibiotic chloramphenicol. Our findings suggest that MGK can serve as a robust tool in functional genomics studies.Keywords
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