Relationship between the number of sperms and the rate of implantation in bitches inseminated into unilateral uterine horn.

Abstract

Studies were made on the number of sperms required for fertilization of ova ovulated from the bilateral ovaries in bitches. The experiment was carried out with 44 female beagles, which were divided into four groups of seven to eleven bitches each. The four groups were inseminated with 40 .times. 106 sperms/0.2 ml, 20 .times. 106 sperms/0.1 ml, 10 .times.106 sperms/0.1 ml, and 3-5.times.106 sperms/0.1 ml, respectively. All the bitches were laparotomized at an optimum time for mating and inseminated at the tip of the horn of an unilateral uterus. In the four group conception took place at a rate of 7/7, 8/8, 10/11, and 2/7, respectively. The number of puppies exceeded the number of ova ovulated on the inseminated side at a rate of 5.7, 6/8, 0/10, and 0/2 in the four groups, respectively. Therefore, it was clarified that then more than 20.times.106 sperms/0.1 ml were inseminated in the ipsilateral uterine horn, ova ovulated on the contralateral side were fertilized also. Then a unilateral ovary was ovariectomized in 11 bitches, which were inseminated with 10.times.106 sperms/0.1 ml in the ipsilateral uterine horn. As a result, only one bitch became conceptive and gave birth to only one puppy. From these results, it was considered that 10.times.106 sperms/0.1 ml may be most adequate for the ova on the side of insemination alone to be fertilized.