Heat-induced accumulation of p53 and hsp72 is suppressed in lung fibroblasts from the SCID mouse
- 1 January 2000
- journal article
- Published by Informa UK Limited in International Journal of Radiation Biology
- Vol. 76 (5), 711-715
- https://doi.org/10.1080/095530000138385
Abstract
To investigate how DNA-dependent protein kinase (DNA-PK) contributes to p53-dependent signal transduction after heat shock, thermosensitivity and accumulation of p53 and hsp72 after heat shock in lung fibroblasts derived from the SCID mouse were analysed. Thermosensitivity at 44 degrees C in colony-forming units and Western blot analysis of p53 and hsp72 were analysed. The results indicated that (1) the thermosensitivity at 44 degrees C of SCID cells was higher than that of parental cells and (2) heat-induced accumulation of p53 and hsp72 was abolished and suppressed in SCID cells as compared with that in parental cells respectively. The findings suggest that the catalytic subunit of DNA-PK may play an important role upstream of p53 and hsp72, which are possible determinants of cellular thermosensitivity.Keywords
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