Protein Dynamics in Iron-Starved Mycobacterium tuberculosis Revealed by Turnover and Abundance Measurement Using Hybrid-Linear Ion Trap-Fourier Transform Mass Spectrometry
- 9 August 2008
- journal article
- research article
- Published by American Chemical Society (ACS) in Analytical Chemistry
- Vol. 80 (18), 6860-6869
- https://doi.org/10.1021/ac800288t
Abstract
To study the proteome response of Mycobacterium tuberculosis H37Rv to a change in iron level, iron-starved late-log-phase cells were diluted in fresh low- and high-iron media containing [15N]-labeled asparagine as the sole nitrogen source for labeling the proteins synthesized upon dilution. We determined the relative protein abundance and protein turnover in M. tuberculosis H37Rv under these two conditions. For measurements, we used a high-resolution hybrid-linear ion trap-Fourier transform mass spectrometer coupled with nanoliquid chromatography separation. While relative protein abundance analysis shows that only 5 proteins were upregulated by high iron, 24 proteins had elevated protein turnover for the cells in the high-iron medium. This suggests that protein turnover is a sensitive parameter to assess the proteome dynamics. Cluster analysis was used to explore the interconnection of protein abundance and turnover, revealing coordination of the cellular processes of protein synthesis, degradation, and secretion that determine the abundance and allocation of a protein in the cytosol and the extracellular matrix of the cells. Further potential utility of the approach is discussed.Keywords
This publication has 50 references indexed in Scilit:
- A Computational Strategy to Analyze Label-Free Temporal Bottom-Up Proteomics DataJournal of Proteome Research, 2008
- Lipid composition and transcriptional response of Mycobacterium tuberculosis grown under iron-limitation in continuous culture: identification of a novel wax esterMicrobiology, 2007
- A New Algorithm Using Cross-Assignment for Label-Free Quantitation with LC−LTQ-FT MSJournal of Proteome Research, 2007
- The gene expression data of Mycobacterium tuberculosis based on Affymetrix gene chips provide insight into regulatory and hypothetical genesBMC Microbiology, 2007
- Quantitative Proteomic and Microarray Analysis of the Archaeon Methanosarcina acetivorans Grown with Acetate versus MethanolJournal of Proteome Research, 2006
- Quantitative Proteomics of the Archaeon Methanococcus maripaludis Validated by Microarray Analysis and Real Time PCRMolecular & Cellular Proteomics, 2006
- Advances in proteomics data analysis and display using an accurate mass and time tag approachMass Spectrometry Reviews, 2006
- Quantitative Proteomic Analysis of Drug-Induced Changes in MycobacteriaJournal of Proteome Research, 2005
- A Mutant of Mycobacterium tuberculosis H37Rv That Lacks Expression of Antigen 85A Is Attenuated in Mice but Retains Vaccinogenic PotentialInfection and Immunity, 2004
- The role of KasA and KasB in the biosynthesis of meromycolic acids and isoniazid resistance in Mycobacterium tuberculosisTuberculosis, 2002