Expression in yeast of a Plasmodium vivax antigen of potential use in a human malaria vaccine.
Open Access
- 31 March 1987
- journal article
- research article
- Published by Rockefeller University Press in The Journal of Experimental Medicine
- Vol. 165 (4), 1160-1171
- https://doi.org/10.1084/jem.165.4.1160
Abstract
DNA coding for 234 amino acids of the circumsporozoite (CS) protein of Plasmodium vivax was incorporated into yeast expression vectors. The DNA encoded all the repeat domain and codons for a highly conserved sequence, KLKQP, found in CS proteins from all malaria parasites. Yeast cells transformed with these autonomously replicating plasmids expressed, upon induction, high levels of the CS polypeptide. The malaria antigen was purified in good yields from yeast extracts and was injected into mice using alum as adjuvant. The antibodies recognized the authentic CS protein, and at high dilutions, they inhibited the invasion of hepatocytes by sporozoites in vitro.This publication has 28 references indexed in Scilit:
- Research Toward Malaria VaccinesScience, 1986
- Immunity to Malaria and Naturally Acquired Antibodies to the Circumsporozoite Protein ofPlasmodium falciparumNew England Journal of Medicine, 1986
- Inhibition of Development of Exoerythrocytic Forms of Malaria Parasites by γ-InterferonScience, 1986
- Circumsporozoite Protein of Plasmodium vivax : Gene Cloning and Characterization of the Immunodominant EpitopeScience, 1985
- Rationale for Development of a Synthetic Vaccine Against Plasmodium falciparum MalariaScience, 1985
- Expression of Plasmodium falciparum Circumsporozoite Proteins in Escherichia coli for Potential Use in a Human Malaria VaccineScience, 1985
- Structure of the Gene Encoding the Immunodominant Surface Antigen on the Sporozoite of the Human Malaria Parasite Plasmodium falciparumScience, 1984
- Structure of the plasmodium knowlesi gene coding for the circumsporozoite proteinCell, 1983
- Identification and chemical synthesis of a tandemly repeated immunogenic region of Plasmodium knowlesi circumsporozoite proteinNature, 1983
- Circumsporozoite proteins of human malaria parasites Plasmodium falciparum and Plasmodium vivax.The Journal of Experimental Medicine, 1982