Variation of osteocyte lacunae size within the tetrapod skeleton: implications for palaeogenomics
- 16 March 2011
- journal article
- research article
- Published by The Royal Society in Biology Letters
- Vol. 7 (5), 751-754
- https://doi.org/10.1098/rsbl.2011.0173
Abstract
Recent studies have emphasized the ability to reconstruct genome sizes (C-values) of extinct organisms such as dinosaurs, using correlations between known genome sizes and bone cell (osteocyte lacunae) volumes. Because of the established positive relationship between cell size and genome size in extant vertebrates, osteocyte lacunae volume is a viable proxy for reconstructing C-values in the absence of any viable genetic material. However, intra-skeletal osteocyte lacunae size variation, which could cause error in genome size estimation, has remained unexplored. Here, 11 skeletal elements of one individual from each of four major clades (Mammalia, Amphibia, Aves, Reptilia) were examined histologically. Skeletal elements in all four clades exhibit significant differences in the average sizes of their lacunae. This variation, however, generally does not cause a significant difference in the estimated genome size when common phylogenetic estimation methods are employed. On the other hand, the spread of the estimations illustrates that this method may not be precise. High variance in genome size estimations remains an outstanding problem. Additionally, a suite of new methods is introduced to further automate the measurement of bone cells and other microstructural features on histological thin sections.Keywords
This publication has 12 references indexed in Scilit:
- Paleogenomic data suggest mammal‐like genome size in the ancestral amniote and derived large genome size in amphibiansJournal of Evolutionary Biology, 2010
- Sauropod dinosaurs evolved moderately sized genomes unrelated to body sizeProceedings Of The Royal Society B-Biological Sciences, 2009
- PhyloPars: estimation of missing parameter values using phylogenyNucleic Acids Research, 2009
- Palaeogenomics of pterosaurs and the evolution of small genome size in flying vertebratesBiology Letters, 2008
- Origin of avian genome size and structure in non-avian dinosaursNature, 2007
- Eukaryotic genome size databasesNucleic Acids Research, 2006
- The Bigger the C-Value, the Larger the Cell: Genome Size and Red Blood Cell Size in VertebratesBlood Cells, Molecules, and Diseases, 2001
- Comparative osteohistology of some embryonic and perinatal archosaurs: developmental and behavioral implications for dinosaursPaleobiology, 2001
- Function of osteocytes in boneJournal of Cellular Biochemistry, 1994
- Size and density of osteocyte lacunae in different regions of long bonesCalcified Tissue International, 1982