Theileria annulata sporozoite surface antigen (SPAG‐1) contains neutralizing determinants in the C terminus
- 1 February 1994
- journal article
- Published by Wiley in Parasite Immunology
- Vol. 16 (2), 97-104
- https://doi.org/10.1111/j.1365-3024.1994.tb00328.x
Abstract
SPAG-1 is a surface antigen on Theileria annulata sporozoites that is a candidate both for inclusion in a subunit vaccine and as a ligand for host cell recognition. We have pinpointed major neutralizing epitopes to the C terminus. To facilitate this we expressed SPAG-1 as a series of defined fragments in the pGEX system. These constructs were validated by sequencing and by their spectrum of reactivity with monoclonal antibody (MoAb) BA4. This MoAb recognizes the elastin motif VGVAPG, that is predicted to occur three times in the N terminal half of SPAG-1. The recombinant proteins were then tested by Western blotting with a neutralizing MoAb (1A7) and two neutralizing bovine sera (10T and 34A). The results demonstrate that 1A7 and the bovine sera react with determinants unique to the C terminus. We mapped the neutralizing determinant recognized by MoAb 1A7 to a 16 residue sequence (residues 807–822) using synthetic peptides. Interestingly the bovine sera do not recognize the 1A7 epitope. The potential role of the C terminus as a ligand for host cell recognition and the implications for sub-unit vaccine production are discussed.Keywords
This publication has 17 references indexed in Scilit:
- Malaria vaccinesCurrent Opinion in Immunology, 1992
- Mimicry of elastin repetitive motifs by Theileria annulata sporozoite surface antigenMolecular and Biochemical Parasitology, 1992
- A recombinant sporozoite surface antigen of Theileria parva induces protection in cattle.Proceedings of the National Academy of Sciences of the United States of America, 1992
- Proteolytic processing of thePlasmodium falciparum merozoite surface protein-1 produces a membrane-bound fragment containing two epidermal growth factor-like domainsMolecular and Biochemical Parasitology, 1991
- Theileria annulata sporozoite surface antigen expressed in Escherichia coli elicits neutralizing antibody.Proceedings of the National Academy of Sciences, 1989
- Bovine theileriosis in IsraelRevue Scientifique et Technique de l'OIE, 1989
- Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferaseGene, 1988
- [19] Rapid and efficient site-specific mutagenesis without phenotypic selectionMethods in enzymology, 1987
- Characterization of biologically active domains on elastin: identification of a monoclonal antibody to a cell recognition siteBiochemistry, 1986
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976