A magneto-DNA nanoparticle system for rapid detection and phenotyping of bacteria
- 5 May 2013
- journal article
- research article
- Published by Springer Science and Business Media LLC in Nature Nanotechnology
- Vol. 8 (5), 369-375
- https://doi.org/10.1038/nnano.2013.70
Abstract
So far, although various diagnostic approaches for pathogen detection have been proposed, most are too expensive, lengthy or limited in specificity for clinical use. Nanoparticle systems with unique material properties, however, circumvent these problems and offer improved accuracy over current methods. Here, we present novel magneto-DNA probes capable of rapid and specific profiling of pathogens directly in clinical samples. A nanoparticle hybridization assay, involving ubiquitous and specific probes that target bacterial 16S rRNAs, was designed to detect amplified target DNAs using a miniaturized NMR device. Ultimately, the magneto-DNA platform will allow both universal and specific detection of various clinically relevant bacterial species, with sensitivity down to single bacteria. Furthermore, the assay is robust and rapid, simultaneously diagnosing a panel of 13 bacterial species in clinical specimens within 2 h. The generic platform described could be used to rapidly identify and phenotype pathogens for a variety of applications.Keywords
This publication has 34 references indexed in Scilit:
- Ultrasensitive Clinical Enumeration of Rare Cells ex Vivo Using a Micro-Hall DetectorScience Translational Medicine, 2012
- Ubiquitous Detection of Gram-Positive Bacteria with Bioorthogonal Magnetofluorescent NanoparticlesACS Nano, 2011
- Miniature magnetic resonance system for point-of-care diagnosticsLab on a Chip, 2011
- Burden of endemic health-care-associated infection in developing countries: systematic review and meta-analysisThe Lancet, 2011
- Bioorthogonal chemistry amplifies nanoparticle binding and enhances the sensitivity of cell detectionNature Nanotechnology, 2010
- Rapid detection and profiling of cancer cells in fine-needle aspiratesProceedings of the National Academy of Sciences, 2009
- Metagenomic Pyrosequencing and Microbial IdentificationClinical Chemistry, 2009
- Bench-to-bedside review: The promise of rapid infection diagnosis during sepsis using polymerase chain reaction-based pathogen detectionCritical Care, 2009
- Then and now: use of 16S rDNA gene sequencing for bacterial identification and discovery of novel bacteria in clinical microbiology laboratoriesClinical Microbiology & Infection, 2008
- Chip–NMR biosensor for detection and molecular analysis of cellsNature Medicine, 2008