Role of Polymer Micelles in the Delivery of Photodynamic Therapy Agent to Liposomes and Cells
Open Access
- 7 February 2020
- Vol. 12 (2), 384
- https://doi.org/10.3390/cancers12020384
Abstract
The use of nanocarriers for hydrophobic photosensitizers, in the context of photodynamic therapy (PDT) to improve pharmacokinetics and bio-distribution, is well-established. However, the mechanisms at play in the internalization of nanocarriers are not well-elucidated, despite its importance in nanocarrier design. In this study, we focus on the mechanisms involved in copolymer poly(ethylene oxide)-block-poly(ε-caprolactone) PEO-PCL and poly(ethylene oxide)-block-poly styrene PEO-PS micelles - membrane interactions through complementary physico-chemical studies on biomimetic membranes, and biological experiments on two-dimensional (2D) and three-dimensional (3D) cell cultures. Förster Resonance Energy Transfer measurements on fluorescently-labelled lipid vesicles, and flow cytometry on two cancerous cell lines enabled the evaluation in the uptake of a photosensitizer, Pheophorbide a (Pheo), and copolymer chains towards model membranes, and cells, respectively. The effects of calibrated light illumination for PDT treatment on lipid vesicle membranes, i.e., leakage and formation of oxidized lipids, and cell viability, were assessed. No significant differences were observed between the ability of PEO-PCL and PEO-PS micelles in delivering Pheo to model membranes, but Pheo was found in higher concentrations in cells in the case of PEO-PCL. These higher Pheo concentrations did not correspond to better performances in PDT treatment. We demonstrated that there are subtle differences in PEO-PCL and PEO-PS micelles for the delivery of Pheo.Keywords
This publication has 46 references indexed in Scilit:
- The journey of a drug-carrier in the body: An anatomo-physiological perspectiveJournal of Controlled Release, 2012
- Killing Hypoxic Cell Populations in a 3D Tumor Model with EtNBS-PDTPLOS ONE, 2011
- Asymmetrical flow field-flow fractionation with multi-angle light scattering and quasi elastic light scattering for characterization of poly(ethyleneglycol-b-ɛ-caprolactone) block copolymer self-assemblies used as drug carriers for photodynamic therapyJournal of Chromatography A, 2011
- Tumor-Selective Delivery of Macromolecular Drugs via the EPR Effect: Background and Future ProspectsBioconjugate Chemistry, 2010
- Light-Triggered Liposomal Release: Membrane Permeabilization by Photodynamic ActionLangmuir, 2009
- Monomeric pheophorbide(a)-containing poly(ethyleneglycol-b-ε-caprolactone) micelles for photodynamic therapyPhotochemical & Photobiological Sciences, 2009
- Nanomedicine—Challenge and PerspectivesAngewandte Chemie International Edition, 2009
- Singlet Oxygen Production in Water: Aggregation and Charge-Transfer EffectsThe Journal of Physical Chemistry, 1996
- Kinetic and equilibrium studies of porphyrin interactions with unilamellar lipidic vesiclesBiochemistry, 1994
- The ATP assay is more sensitive than the succinate dehydrogenase inhibition test for predicting cell viabilityEuropean Journal of Cancer and Clinical Oncology, 1987