Light-Triggered Liposomal Release: Membrane Permeabilization by Photodynamic Action
- 10 December 2009
- journal article
- research article
- Published by American Chemical Society (ACS) in Langmuir
- Vol. 26 (8), 5726-5733
- https://doi.org/10.1021/la903867a
Abstract
Photosensitized damage to liposome membranes was studied by using different dye-leakage assays based on fluorescence dequenching of a series of dyes upon their release from liposomes. Irradiation of liposomes with red light in the presence of a photosensitizer, trisulfonated aluminum phthalocyanine (AlPcS3), resulted in the pronounced leakage of carboxyfluorescein, but rather weak leakage of sulforhodamine B and almost negligible leakage of calcein from the corresponding dye-loaded liposomes. The same series of selectivity of liposome leakage was obtained with chlorin e6 that appeared to be more potent than AlPcS3 in bringing about the photosensitized liposome leakage. Electrically neutral zinc phthalocyanine tetrasubstituted with a glycerol moiety (ZnPcGlyc4) was less effective than negatively charged AlPcS3 in provoking the light-induced liposome permeabilization. On the contrary, both ZnPcGlyc4 and AlPcS3 were much more effective than chlorin e6 in sensitizing gramicidin channel inactivation in planar bilayer lipid membranes, thus showing that relative photodynamic efficacy of sensitizers can differ substantially for damaging different membrane targets. The photosensitized liposome permeabilization was apparently associated with oxidation of lipid double bonds by singlet oxygen as evidenced by the mandatory presence of unsaturated lipids in the membrane composition for the photosensitized liposome leakage to occur and the sensitivity of the latter to sodium azide. The fluorescence correlation spectroscopy measurements revealed marked permeability of photodynamically induced pores in liposome membranes for such photosensitizer as AlPcS3.This publication has 83 references indexed in Scilit:
- Near‐IR Remote Release from Assemblies of Liposomes and NanoparticlesAngewandte Chemie International Edition, 2009
- Remotely Triggered Liposome Release by Near-Infrared Light Absorption via Hollow Gold NanoshellsJournal of the American Chemical Society, 2008
- Nanocarriers as an emerging platform for cancer therapyNature Nanotechnology, 2007
- Hyperthermia mediated liposomal drug deliveryInternational Journal of Hyperthermia, 2006
- The Role of Metal Nanoparticles in Remote Release of Encapsulated MaterialsNano Letters, 2005
- Functional Consequences of Oxidative Membrane DamageThe Journal of Membrane Biology, 2005
- Light-Responsive Polyelectrolyte/Gold Nanoparticle MicrocapsulesThe Journal of Physical Chemistry B, 2005
- Secreted phospholipase A2 as a new enzymatic trigger mechanism for localised liposomal drug release and absorption in diseased tissueBiochimica et Biophysica Acta (BBA) - Biomembranes, 2002
- PHOTOSENSITIZED LYSIS OF LIPOSOMES BY HEMATOPORPHYRIN DERIVATIVEPhotochemistry and Photobiology, 1983
- TYPE I AND TYPE II MECHANISMS IN THE PHOTOSENSITIZED LYSIS OF PHOSPHATIDYLCHOLINE LIPOSOMES BY HEMATOPORPHYRINPhotochemistry and Photobiology, 1982