Extended-gate field-effect transistor packed in micro channel for glucose, urea and protein biomarker detection
- 9 November 2015
- journal article
- Published by Springer Science and Business Media LLC in Biomedical Microdevices
- Vol. 17 (6), 1-9
- https://doi.org/10.1007/s10544-015-0020-4
Abstract
This study developed a packaging method to integrate the extended-gate field-effect transistor (EGFET) into a microfluidic chip as a biological sensor. In addition, we present two immobilization approaches for the bio-recognition that are appropriate to this chip, allowing it to measure the concentrations of hydrogen ions, glucose, urea, and specific proteins in a solution. Alginate-calcium microcubes were used to embed the enzymes and magnetic powder (enzyme carrier). When the sensing chip needs the enzyme for the catalytic reaction, the alginate microcubes containing the corresponding enzymes enter through the flow channel and are immobilized on the EGFET surface with an external magnet. High sensing performance of the chip is achieved, with 37.45 mV/mM for measuring hydrogen ions at pH 6–8 with a linearity of 0.9939, 7.00 mV/mM for measuring glucose with a linearity of 0.9962, and 8.01 mV/mM for measuring urea with a linearity of 0.9809. In addition, based on the principle of the immunoassay, the magnetic beads with the specific antibody were used to capture the target protein in the sample. Then, negatively charged DNA fragments bound to a secondary antibody were used to amplify the signal for EGFET measurement. The magnetic beads with completed immune response bonding were then fixed on the surface of the sensor by an external magnetic field. Therefore, the measured object can directly contact the sensor surface, and quantitative detection of the protein concentration can be achieved. Apolipoprotein A1 (APOA1) was detected as a target protein, with a minimum detection limit of approximately 12.5 ng/mL.Keywords
This publication has 21 references indexed in Scilit:
- Multiplexed quantification of 63 proteins in human urine by multiple reaction monitoring-based mass spectrometry for discovery of potential bladder cancer biomarkersJournal of Proteomics, 2012
- Identification of Apo-A1 as a biomarker for early diagnosis of bladder transitional cell carcinomaProteome Science, 2011
- Fabrication of stable antibody-modified field effect transistors using electrical activation of Schiff base cross-linkages for tumor marker detectionBiosensors and Bioelectronics, 2010
- Cantilever biosensorsThe Analyst, 2008
- Immobilization of E. coli bacteria in three-dimensional matrices for ISFET biosensor designBioelectrochemistry, 2007
- Integrated microfluidic systems for automatic glucose sensing and insulin injectionSensors and Actuators B: Chemical, 2007
- Rapid and simple detection of food poisoning bacteria by bead assay with a microfluidic chip-based systemJournal of Microbiological Methods, 2006
- Physical adsorption of vapors — three personaeColloids and Surfaces A: Physicochemical and Engineering Aspects, 1996
- A generalized theory of an electrolyte-insulator-semiconductor field-effect transistorIEEE Transactions on Electron Devices, 1986
- Development of an Ion-Sensitive Solid-State Device for Neurophysiological MeasurementsIEEE Transactions on Biomedical Engineering, 1970