Binary Solutions of Hyaluronan and Lactose-Modified Chitosan: The Influence of Experimental Variables in Assembling Complex Coacervates
Open Access
- 12 April 2020
- Vol. 12 (4), 897
- https://doi.org/10.3390/polym12040897
Abstract
A miscibility study between oppositely charged polyelectrolytes, namely hyaluronic acid and a lactose-modified chitosan, is here reported. Experimental variables such as polymers’ weight ratios, pH values, ionic strengths and hyaluronic acid molecular weights were considered. Transmittance analyses demonstrated the mutual solubility of the two biopolymers at a neutral pH. The onset of the liquid-liquid phase separation due to electrostatic interactions between the two polymers was detected at pH 4.5, and it was found to be affected by the overall ionic strength, the modality of mixing and the polymers’ weight ratio. Thorough Dynamic Light Scattering (DLS) measurements were performed to check the quality of the formed coacervates by investigating their dimensions, homogeneity and surface charge. The whole DLS results highlighted the influence of the hyaluronic acid molecular weight in affecting coacervates’ dispersity and size.Keywords
Funding Information
- Interreg (1472551605)
This publication has 40 references indexed in Scilit:
- Complex coacervation: Principles, mechanisms and applications in microencapsulationInternational Journal of Biological Macromolecules, 2018
- Review on plant protein–polysaccharide complex coacervation, and the functionality and applicability of formed complexesJournal of the Science of Food and Agriculture, 2018
- Complex coacervation in charge complementary biopolymers: Electrostatic versus surface patch bindingAdvances in Colloid and Interface Science, 2017
- Chitosan/Hyaluronic Acid Nanoparticles: Rational Design Revisited for RNA DeliveryMolecular Pharmaceutics, 2017
- Protein Encapsulation via Polypeptide Complex CoacervationACS Macro Letters, 2014
- Loading and Protection of Hydrophilic Molecules into Liposome-Templated Polyelectrolyte NanocapsulesLangmuir, 2014
- Hyaluronic acid-coated chitosan nanoparticles: Molecular weight-dependent effects on morphology and hyaluronic acid presentationJournal of Controlled Release, 2013
- Templated globules — applications and perspectivesAdvances in Colloid and Interface Science, 2013
- Complex coacervation of pea protein isolate and alginate polysaccharidesFood Chemistry, 2012
- Complex coacervation between β-lactoglobulin and acacia gum in aqueous mediumFood Hydrocolloids, 1999