Purification and Characterization of Bovine Brain 5′‐Nucleotidase
- 5 October 1982
- journal article
- Published by Wiley in Journal of Neurochemistry
- Vol. 39 (4), 982-989
- https://doi.org/10.1111/j.1471-4159.1982.tb11486.x
Abstract
The 5′-nucleotidase located in the cytoplasmic fraction of bovine brain cortex was purified to electrophoretic homogeneity. The molecular weight was 134,000 daltons in the presence of sodium deoxycholate, whereas the enzyme formed high molecular weight aggregates in the absence of detergent. The purified enzyme showed the same kinetic and electrophoretic behaviour as the enzyme present in the original cytoplasmic fraction, and the presence of surfactants did not change the Km and Vm, values. The nucleotidase from this source was a phosphohydrolase of 5′-mononucleotides acting on the deoxyribonucleotides and ribonucleotides of purines and pyrimidines. 5′-IMP was the preferred substrate; the optimum pH was 7.5. The study of the influence of the temperature on the initial reaction rates allowed calculation of the δEa, and δHo values. The variation of Vm and Km with a change in pH suggests the existence of a sulfhydryl group and an imidazole group in the enzymesubstrate complex.Keywords
This publication has 32 references indexed in Scilit:
- Concanavalin a inhibits the interaction of snake venom 5′‐nucleotidase and actinFEBS Letters, 1979
- Phospholipid composition of plasma membranes and kinetic properties of membrane-bound nucleotidase from marine bacteriaInternational Journal of Biochemistry, 1978
- Purification and Properties of 5′‐Nucleotidase from Lymphocyte Plasma MembranesEuropean Journal of Biochemistry, 1978
- Detergents modify the form of arrhenius plots of 5′‐nucleotidase activityFEBS Letters, 1978
- Characterization of HeLa 5′-Nucleotidase: A Stable Plasma Membrane MarkerMembrane Biochemistry, 1978
- Differences in the Modulations of the Soluble or Plasma‐Membrane‐Bound 5′‐NucleotidaseEuropean Journal of Biochemistry, 1977
- Activité 5′-nucléotidase des membranes plasmiques de lymphocytes. Effet de la concanavaline ABiochimie, 1977
- Effect of sodium deoxycholate on 5′-nucleotidaseBiochimica et Biophysica Acta (BBA) - Biomembranes, 1976
- 5'-Nucleotidase from smooth muscle of small intestine and from brain. Inhibition by nucleotidesBiochemistry, 1975
- 5′-ribonucleotide phosphohydrolase from bovine pituitary glandBiochimica et Biophysica Acta (BBA) - Enzymology and Biological Oxidation, 1966