Frozen‐Thawed Mononuclear Cells as a Source of B Lymphocytes for Antibody Screening

Abstract

B lymphocytes were isolated from fresh blood and frozen‐thawed lymphocyte preparations from the same donors. A panel of 55 human antisera, previously found to react with human peripheral blood B lymphocytes in cytotoxicity assays, was screened against these B‐cell preparations. There was essentially no difference in the reactions of B‐cell antisera with fresh B lymphocytes when compared with frozen‐thawed cells. The percentage recovery of B cells from the fresh and frozen preparations was not significantly different.