Microcantilever biosensors based on conformational change of proteins
- 25 February 2008
- journal article
- review article
- Published by Royal Society of Chemistry (RSC) in The Analyst
- Vol. 133 (4), 434-443
- https://doi.org/10.1039/b713330h
Abstract
Microcantilevers (MCLs) hold a position as a cost-effective and highly sensitive sensor platform for medical diagnostics, environmental analysis and fast throughput analysis. MCLs are unique in that adsorption of analytes on the microcantilever (MCL) surface changes the surface characteristics of the MCL and results in bending of the MCL. Surface stress due to conformation change of proteins and other polymers has been a recent focus of MCL research. Since conformational changes in proteins can be produced through binding of anylates at specific receptor sites, MCLs that respond to conformational change induced surface stress are promising as transducers of chemical information and are ideal for developing microcantilever-based biosensors. The MCL can also potentially be used to investigate conformational change of proteins induced by non-binding events such as post-translational modification and changes in temperature or pH. This review will provide an overview of MCL biosensors based on conformational change of proteins bound to the MCL surface. The models include conformational change of proteins, proteins on membranes, enzymes, DNA and other polymers.Keywords
This publication has 48 references indexed in Scilit:
- Cantilever transducers as a platform for chemical and biological sensorsReview of Scientific Instruments, 2004
- Detection of CrO42- Using a Hydrogel Swelling Microcantilever SensorAnalytical Chemistry, 2003
- Micromechanical cantilever-based biosensorsSensors and Actuators B: Chemical, 2001
- Microcantilever-based biosensorsUltramicroscopy, 2000
- Measuring Surface-Induced Conformational Changes in ProteinsLangmuir, 1999
- MICROCANTILEVER SENSORSMicroscale Thermophysical Engineering, 1997
- Resonance response of scanning force microscopy cantileversReview of Scientific Instruments, 1994
- Thermal and ambient-induced deflections of scanning force microscope cantileversApplied Physics Letters, 1994
- Observation of a chemical reaction using a micromechanical sensorChemical Physics Letters, 1994
- The tension of metallic films deposited by electrolysisProceedings of the Royal Society of London. Series A, Containing Papers of a Mathematical and Physical Character, 1909