Localization of transglutaminase-reactive glutamine residues in bovine osteopontin
- 15 November 1994
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 304 (1), 13-16
- https://doi.org/10.1042/bj3040013
Abstract
Here we report the identification of two transglutaminase-reactive glutamines (Gln-34 and Gln-36) in bovine osteopontin (OPN). Sequence alignment revealed that these glutamines are conserved in all known OPN sequences, indicating a functional importance of this region of the protein. Furthermore, immunological analysis of bovine bone demonstrated that OPN is present in high-molecular-mass complexes in vivo. These findings support the functional aspects of a transglutaminase-catalysed cross-linking of OPN in facilitating cellular attachment and tissue calcification.Keywords
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