Invitro plant regeneration via callus culture of mature Salixexigua
- 1 December 1989
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Forest Research
- Vol. 19 (12), 1634-1638
- https://doi.org/10.1139/x89-247
Abstract
Callus induction, callus growth, and plantlet regeneration were examined using leaf explants of three Salix exigua clones. Calli were initiated on three basal media supplemented with 0.1 mg/L (0.44 .mu.M) or 0.5 mg/L (2.2 .mu.M) of benzylaminopurine and 0.1 mg/L (0.45 .mu.M) or 0.5 mg/L (2.3 .mu.M) of 2,4-dichlorophenoxyacetic acid in a factorial fashion. After 7 weeks of growth, callus production was highest on woody plant medium. Developed calli were subsequently cultured on woody plant medium supplemented with either 0.1 or 0.5 mg/L of benzylaminopurine for shoot induction. Shoot primordia developed only at 0.1 mg/L (0.44 .mu.M) benzylaminopurine in two clones, suggesting clonal variation in organogenic responses. Shoots larger than 1.0 cm in length were successfully rooted in half-strength Murashige and Skoog medium without hormones.This publication has 5 references indexed in Scilit:
- European HardwoodsPublished by Springer Nature ,1987
- Effects of N6-benzyladenine on shoots of five willow clones (Salix spp.) cultured in vitroPlant Cell, Tissue and Organ Culture (PCTOC), 1985
- Micropropagation method for a hybrid willow (Salix matsudana×albaNZ-1002)New Zealand Journal of Botany, 1980
- A synopsis of American SalixCanadian Journal of Botany, 1976
- A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue CulturesPhysiologia Plantarum, 1962