Development of morphologic heterogeneity of hepatocyte mitochondria in the mouse

Abstract

The ultrastructure of mitochondria in perioportal and perihepatic hepatocytes from newborn, 5‐, 10‐, and 20‐day‐old, and adult male ddY mice was analyzed by quantitative electron microscopy. In newborn and 5‐ and 10‐day‐old animals, the axial ratio (length per diameter), surface to volume ratio (area of the outer membrane per unit mitochondrial volume), and volume density were not significantly different between periportal and periphepatic cells. In 20‐day‐old and adult animals, the surface to volume ratio was greater in perihepatic cells than periportal cells, and the volume density was greater in periportal cells than perihepatic cells. The axial ratio became greater in perihepatic cells than periportal cells in adult animals. However, there were no differences in the surface density of the outer membrane, and of the inner membrane and cristae between the cells of both zones in all age groups examined. When the data were expressed as volume and area per cell, the patterns of subacinar distribution and age‐related changes differed from the patterns seen in the volume and surface density data mainly in adult animals. This difference was generally caused by the marked increase in hepatocyte volume between 20 days of age and adulthood, especially in perihepatic cells. The results show that differences between mitochondria in periportal cells and those in perihepatic cells in the shape (the axial and surface to volume ratios), volume density, and area of the outer membrane per cell, evident in adult animals, are not present in newborn animals but arise during postnatal development.