Bienzyne Electrodes for ATP, NAD+, Starch and Disaccharides Based on a Glucose Sensor

Abstract

A glucose measuring device based on the oxidation of glucose by glucose oxidase and an amperometric kinetic detection was developed. The characteristics obtained with this instrument are comparable with the present glucose instruments but the stability of the enzyme membrane is better and the measuring frequency is higher. In order to expand the applicability of this device to other substrates there was developed a family of bioenzyme electrodes. Enzymes producing glucose as enzymes consuming glucose in addition to glucose oxidase were used. For determination of peroxidase substrates besides a peroxidase-catalase electrode a three-enzyme system consisting of glucose oxidase, peroxidase and catalase was used.