DETERMINATION OF Δ4-3-KETOSTEROIDS BY MEASUREMENT OF THE ABSORBANCE OF THEIR 2,4-DINITROPHENYLHYDRAZONES
- 1 September 1957
- journal article
- Published by Canadian Science Publishing in Canadian Journal of Biochemistry and Physiology
- Vol. 35 (9), 699-710
- https://doi.org/10.1139/o57-082
Abstract
A rapid, sensitive method suitable for the quantitative determination of ethisterone, methyltestosterone, testosterone, and progesterone is described. The method involves the extraction of the 2,4-dinitrophenylhydrazones of the steroid from the excess dinitrophenylhydrazine reagent with cyclohexane and measurement of the absorbance of the resulting yellow cyclohexane extract. The method has been used in the analysis of a number of tablets and aqueous suspensions containing a mixture of one of these Δ4-3-ketosteroids and other ingredients.Keywords
This publication has 3 references indexed in Scilit:
- Isonicotinic Acid Hydrazide as Reagent for Determination of Δ4-3-KetosteroidsAnalytical Chemistry, 1955
- Forest Service Observes Its Golden AnniversaryScience, 1955
- Chromatographic Isolation and Estimation of the Natural Œstrogens from TissueNature, 1952