Primary structure of rat liver elongation factor 2 deduced from the cDNA sequence

Abstract

A number of cDNA clones in summary encoding 700 amino acid residues from the N‐end of rat liver elongation factor 2 (EF‐2) and including 49 nucleotides of the 5′‐untranslated mRNA region have been obtained. EF‐2 cDNA clones were isolated from gradually constructed small (1000–5000 clones) specific cDNA libraries using the primer extension method for synthesis of the first cDNA chain. The complete primary structure of cDNA and protein EF‐2 from rat liver was derived taking into account the primary structure of the 3′‐terminal region EF‐2 cDNA previously reported [(1986) Proc. Natl. Acad. Sci. USA 83, 4978–4982]. Comparison of this cDNA with hamster cDNA has shown that (i) the base sequences had a 89.7% homology while that of the 5′‐untranslated region was 73%; (ii) there are two amino acid replacements in rat liver EF‐2 as compared with hamster EF‐2.